Molecular Evolutionary Analysis of Classical Swine Fever Virus

作者：Zhang, H.^*; Wang, Y. H.; Wu, Z. J.; Cui, Y. D.

来源：Israel Journal of Veterinary Medicine, 2013, 68(1): 43-47.

• Classical swine fever virus
• Envelope glycoprotein E2
• Molecular evolutionary

The E2 glycoprotein gene of cases of 15 classical swine fever virus (CSFV) were isolated from HeiLongJiang province of China from 2011 to 2012 were amplified by RT-PCR. A 190 nt amplicon of E2 gene was sequenced and compared with 7 CSFV reference strains. Phylogenetic analysis of 190 nucleotides of E2 gene indicated that 15 HeiLongJiang isolates was classified into subgroup 1.1, which indicated that the CSFV Group 1 were still contributing to the epidemic of classical swine fever (CSF) in HeiLongJiang province of China. We synchronously performed Bayesian Markov chain Monte Carlo (MCMC) analysis of the E2 gene region to investigate the diversity of evolutionary rates between Group 1 and Group 2 of CSFV. The results revealed that the mean evolutional rate of Group 1 and 2 were 5.88 x 10(-4) and 3.04 x 10(-3) substitutions per site per year, respectively, which demonstrated that Group 2 evolved much faster than Group 1. The striking differences in evolutionary rates of two genotypes likely implied that both of them had their different evolutionary strategies.

• 出版日期2013-3
• 单位黑龙江八一农垦大学