Gene Expression and Regulation of Wax Moth Transferrin by PAMPs and Heavy Metals

作者：Han Jikhyon; Lee Ji Sook; Lee Chang Seok; Koh Sang Kyun; Seo Sook Jae; Yun Chi Young^*

来源：Animal Cells and Systems, 2009, 13(3): 297-304.

DOI：10.1080/19768354.2009.9647222

• transferrin
• wax moth
• cDNA cloning
• pathogen-associated molecular pattern
• PAMP
• iron
• cadmium

A complete mRNA sequence of transferrin from the wax moth, Galleria mellonella, was obtained, and compared with those of other species. We previously reported that the sequence was most similar to those of Manduca sexta and Bombyx mori. As in other moths, G mellonella transferrin had only one iron-binding site at its N-terminal region. Semi-qRT PCR was conducted to investigate tissue-specific distribution and transcriptional regulation of the wax moth transferrin mRNA. Larval muscle and fat body contained larger quantity of mRNA than other tested tissues. In this study, it was observed that iron and cadmium regulated transferrin transcription, and this regulation pattern was tissue specific. Iron up-regulated transferrin mRNA level in fat body, while suppressed it in the Malpighian tubules and silk glands. Cadmium decreased the mRNA level in fat body, muscle, and Malpighian tubules, but significantly increased the mRNA level in silk glands. In addition, the mRNA expression was induced by all tested pathogen-associated molecular patterns (PAMPs) including LIPS, lipoteichoic acid (LTA), glucan, and even chitin.

• 出版日期2009-9-30