Adipose tissue hyperplasia with enhanced adipocyte-derived stem cell activity in Tc1(C8orf4)-deleted mice

作者：Jang Hayoung; Kim Minsung; Lee Soyoung; Kim Jungtae; Woo Dong Cheol; Kim Kyung Won; Song Kyuyoung; Lee Inchul

来源：Scientific Reports, 2016, 6(1): 35884.

DOI：10.1038/srep35884

Adipose tissue hyperplasia with increased number of adipocytes is implicated in a protective rather than deleterious effect on obesity-associated metabolic disorder. It is poorly understood how the adipose tissue cellularity is regulated. Tc1 is a gene of vertebrates that regulates diverse downstream genes. Young Tc1-deleted mice fed on standard chow diet show expanded adipose tissue with smaller adipocytes in size compared to wild type controls, representing adipose tissue hyperplasia. Tc1(-/-) mice show enhanced glucose tolerance and reduced serum lipids. Adipocyte-derived stem cells (ADSCs) from Tc1(-/-) mice show enhanced proliferative and adipogenic capacity compared to wild type controls, suggesting that the adipose hyperplasia is regulated at the stem cell level. PPAR gamma and CEBP alpha are up-regulated robustly in Tc1(-/-) ADSCs upon induction for adipogenesis. Wisp2 and Dlk1, inhibitors of adipogenesis, are down-regulated in Tc1(-/-) ADSCs compared to controls. Tc1-transfected NIH3T3 cells show higher beta-catenin reporter signals than vector transfected controls, suggesting a role of canonical Wnt signaling in the Tc1-dependent adipose regulation. Our data support that Tc1 is a novel regulator for adipose stem cells. Adipose tissue hyperplasia may be implicated in the metabolic regulation of Tc1(-/-) mice.

• 出版日期2016-10-24