Activated platelet-derived growth factor beta receptor and Ras-mitogen-activated protein kinase pathway in natural bovine urinary bladder carcinomas

作者：Corteggio Annunziata; Di Geronimo Ornella; Roperto Sante; Roperto Franco; Borzacchiello Giuseppe^*

来源：Veterinary Journal, 2012, 191(3): 393-395.

DOI：10.1016/j.tvjl.2011.03.019

• Bovine papillomavirus
• Platelet-derived growth factor
• Urinary bladder tumours

Bovine papillomavirus types 1 or 2 (BPV-1/2) are involved in the aetiopathogenesis of bovine urinary bladder cancer. BPV-1/2 E5 activates the platelet-derived growth factor 0 receptor (PDGF beta 2). The aim of this study was to analyse the Ras/mitogen-activated protein kinase (MAPK) pathway in relation to activation of PDGF beta R in natural bovine urinary bladder carcinomas. Co-immunoprecipitation and Western blot analysis demonstrated that recruitment of growth factor receptor bound protein 2 (GRB-2) and Sos-1 to the activated PDGF beta R was increased in carcinomas compared to normal tissues. Higher grade bovine urinary bladder carcinomas were associated with activation of Ras, but not with activation of downstream mitogen-activated protein kinase/extracellular signal-regulated kinase (Mek 1/2) or extracellular signal-regulated kinase (Erk 1/2).

• 出版日期2012-3