A novel glycoprotein from mushroom Hypsizygus marmoreus (Peck) Bigelow with growth inhibitory effect against human leukaemic U937 cells

作者：Tsai Pei Feng; Ma Chia Yu; Wu James Swi Bea^*

来源：Food Chemistry, 2013, 141(2): 1252-1258.

DOI：10.1016/j.foodchem.2013.04.024

• Hypsizygus marmoreus
• Glycoprotein
• HM-3A
• Tumour
• Leukaemic cell
• Growth inhibitory effect

This study was to isolate the anti-leukaemic component from edible mushroom Hypsizygus marmoreus (Peck) Bigelow. Crude protein was extracted from the basidioma, and then purified with DEAE-Sepharose CL-6B ion exchange chromatography followed by Sephacryl S-300 gel filtration. A protein which exerted high growth inhibitory effect on human leukaemic U937 cells and sufficient toxicological safety on normal human white blood cells was isolated and named HM-3A. Electrophoresis showed HM-3A approximately 52 kDa in size. N-terminal analysis found the amino acid sequence ATTQWKTSAA and confirmed HM-3A a novel protein. High-performance anion-exchange column chromatography revealed HM-3A a glycoprotein with galactose as the major monosaccharide. Haemagglutination assay proved it non-lectin. We suggest that HM-3A is worth further investigation for antitumour use.

• 出版日期2013-11-15