Anaplasma phagocytophilum infection induces apoptosis in HL-60 cells

作者：Karki Pratap^*; Ijdo Jacob W

来源：World Journal of Microbiology and Biotechnology, 2011, 27(11): 2741-2746.

DOI：10.1007/s11274-011-0745-z

• Anaplasma
• Apoptosis
• HL-60
• Neutrophils
• Anaplasmosis

Anaplasma phagocytophilum, the causative agent of human granulocytic anaplasmosis, is an obligate intracellular bacterium that survives in neutrophils by delaying apoptosis. The human promyelocytic leukemia cell line HL-60 has been the ultimate choice for culturing Anaplasma in vitro. In this study, we assessed the various events of drug-induced apoptosis in A. phagocytophilum-infected HL-60 cells. Anaplasma infection reduced the cell viability and increased the apoptosis in HL-60 cells and staurosporine or etoposide-induced apoptosis was further exacerbated with Anaplasma infection. Altogether our results suggest that A. phagocytophilum infection is proapoptotic in HL-60 cells unlike in neutrophils where it is antiapoptotic.

• 出版日期2011-11