A distinct class of small RNAs arises from pre-miRNA-proximal regions in a simple chordate

作者：Shi Weiyang; Hendrix David; Levine Mike^*; Haley Benjamin

来源：Nature Structural & Molecular Biology, 2009, 16(2): 183-189.

DOI：10.1038/nsmb.1536

MicroRNAs (miRNAs) have been implicated in various cellular processes. They are thought to function primarily as inhibitors of gene activity by attenuating translation or promoting mRNA degradation. A typical miRNA gene produces a predominant similar to 21-nucleotide (nt) RNA (the miRNA) along with a less abundant amiRNA* product. We sought to identify miRNAs from the simple chordate Ciona intestinalis through comprehensive sequencing of small RNA libraries created from different developmental stages. Unexpectedly, half of the identified miRNA loci encode up to four distinct, stable small RNAs. The additional RNAs, miRNA-offset RNAs (moRs), are generated from sequences immediately adjacent to the predicted similar to 60-nt pre-miRNA. moRs seem to be produced by RNAse III-like processing, are similar to 20 nt long and, like miRNAs, are observed at specific developmental stages. We present evidence suggesting that the biogenesis of moRs results from an intrinsic property of the miRNA processing machinery in C. intestinalis.

• 出版日期2009-2