Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi

作者：Tomida Junko^*; Oumi Ayako; Okamoto Tatsuya; Morita Yuji; Okayama Akihiko; Misawa Naoaki; Hayashi Tetsuya; Akaike Takaaki; Kawamura Yoshiaki

来源：Microbiology and Immunology, 2013, 57(5): 353-358.

DOI：10.1111/1348-0421.12044

• antimicrobial susceptibility
• broth microdilution method
• Helicobacter cinaedi

The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.

• 出版日期2013-5