Neoechinulin A suppresses amyloid-beta oligomer-induced microglia activation and thereby protects PC-12 cells from inflammation-mediated toxicity

作者：Dewapriya Pradeep; Li Yong Xin; Himaya S W A; Pangestuti Ratih; Kim Se Kwon^*

来源：NeuroToxicology, 2013, 35: 30-40.

DOI：10.1016/j.neuro.2012.12.004

• Neoechinulin A
• Alzheimer's disease
• Amyloid-beta
• Neuroinflammation
• Reactive oxygen species
• Pro-inflammatory mediators

A pathological hallmark of Alzheimer's disease (AD), aggregation and deposition of amyloid-beta peptides, has been recognized as a potent activator of microglia-mediated neuroinflammation and neuronal dysfunction. Therefore, downregulation of microglial activation has a significant therapeutic demand. In this study, focus was given to evaluate the ability of neoechinulin A, an indole alkaloid isolated from marine-derived Microsporum sp., to attenuate microglial activation by oligomeric amyloid-beta 1-42 (A beta 42). Neoechinulin A treatment significantly inhibited the generation of reactive oxygen and nitrogen species in A beta 42-activated BV-2 microglia cells. In addition, we found that neoechinulin A significantly suppressed the production of neurotoxic inflammatory mediator tumour necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and prostaglandin E-2 (PGE(2)) in activated BV-2 cells. Moreover, the treatment downregulated the protein and gene expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-alpha, IL-1 beta and IL-6. Further, activated microglia-mediated apoptosis of PC-12 pheochromocytoma cells was significantly repressed by neoechinulin A. The molecular mechanism studies suggested that neoechinulin A may block the phosphorylation of mitogen-activated protein kinase (MAPK) molecule p38, apoptosis signal-regulating kinase 1 (ASK-1) and nuclear translocation of nuclear factor-kappa B (NF-kappa B) p65 and p50 subunits. Regulation of these signalling pathways have most probably contributed to the anti-inflammatory activity of neoechinulin A. Collectively, these results suggest that with further studies neoechinulin A have a potential to be developed as a modulator of neuroinflammatory process in AD.

• 出版日期2013-3