A Post-translational Cyclodehydratase, PatD, Tolerates Sequence Variation in the C-terminal Region of Substrate Peptides

作者：Goto Yuki^*; Suga Hiroaki^*

来源：Chemistry Letters, 2016, 45(11): 1247-1249.

DOI：10.1246/cl.160562

• Post-translational modification
• Cyclodehydratase
• Azoline-containing peptides

A mutagenesis study of the downstream recognition sequence (dRS) in the substrate peptide of a post-translational cyclodehydratase, PatD, was conducted by means of an in vitro reconstituted translation system integrated with PatD, named the FIT-PatD system. The results have revealed that PatD tolerates various dRS sequences, enabling the one-pot biosynthesis of azoline-containing peptides (Az-peptides) with various C-terminal tag sequences such as hexahistidine, FLAG epitope, and Avi-tag.

• 出版日期2016-11