科研之友
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摘要
Hair follicle stem cell can differentiate into the epithelial cells of hair follicles and be involved in the formation of epidermis, and sebaceous glands. Thus, hair follicle stem cell is an ideal seed cell for tissue-engineered skin. In this study, hair follicle fibroblasts and hair follicle stem cells had been separated from vibrissae of rats. The hair follicle fibroblasts were positive for laminin and fibronectin. Hair follicle stem cells were identified by the expression of cytokeratin 15 and 19. PELNAC scaffold was combined with hair follicle fibroblasts to construct artificial dermis, and hair follicle stem cells were seeded on artificial dermis to construct tissue-engineered skin. The tissue-engineered skin was grafted into the full-thickness skin defects of BALB/c-nu mice. Epidermal growth factor was incorporated into the transplanted tissue-engineered skin. And it stimulates keratinocyte proliferation and migration during wound repair. The results of this study showed that epidermal growth factor reduced the inflammatory response, promoted the secretion of extracellular matrix from fibroblasts, and improved the wound microenvironment, and accelerated wound healing and re-epithelialization. From these, we can conclude that epidermal growth factor accelerates the repair of full-thickness skin defects covered by hair follicle-stem-cell-seeded tissue-engineered skin.
- 出版日期2015-7
- 单位广州中医药大学
