DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex

作者：Jang Soo Hwa; Kim Ah Ram; Park Neung Hwa; Park Jeong Woo^*; Han In Seob^*

来源：Molecules and Cells, 2016, 39(9): 699-704.

DOI：10.14348/molcells.2016.0149

• cyclin B/Cdk1 complex
• DRG2
• G2/M check point
• p21

Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [H-3] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but up-regulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.

• 出版日期2016-9-30