Map-based cloning of the ALK gene, which controls the gelatinization temperature of rice

作者：Gao, ZY; Zeng, DL; Cui, X; Zhou, YH; Yan, M; Huang, D; Li, JY; Qian, Q^*

来源：Science China Life Sciences, 2003, 46(6): 661-668.

DOI：10.1360/03yc0099

• rice (Oryza sativa L.)
• ALK
• soluble starch synthase II
• gelatinization temperature
• map-based cloning

Gelatinization temperature (GT) is an important parameter for evaluating the cooking and eating quality of rice besides amylose content (AC). The inheritance of the genes affecting GT has been widely studied and is considered to be controlled by a major gene. Here, we report the map-based cloning of rice ALK that encodes the soluble starch synthase 11 (SSSII). Comparison between the DNA sequences from different rice varieties, together with the results obtained with digestion of the rice seeds in alkali solution, indicates that the base substitutions in coding sequence of ALK may cause the alteration in GT.

• 出版日期2003-12
• 单位中国水稻研究所; 中国科学院遗传与发育生物学研究所