Nanoparticle realgar powders induce apoptosis in U937 cells through caspase MAPK and mitochondrial pathways

作者：Wang Xiao bo; Gao Hui yuan; Hou Bai ling; Huang Jiian; Xi Rong gang; Wu Li jun^*

来源：Archives of Pharmacal Research, 2007, 30(5): 653-658.

DOI：10.1007/BF02977662

• realgar
• apoptosis
• Bcl-2/Bax
• caspase
• MAPK

Nanoparticle realgar powders (NRP) inhibited U937 cell growth in a time and dose-dependent manner. U937 cells treated with NRP showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Caspase family inhibitor (z-VAD-fmk), caspase-8, -9 inhibitor (z-IETD-fmk, Ac-LEHD-CHO, respectively) and caspase-3 inhibitor (zDEVD-fmk) partially prevented NRP -induced apoptosis. Moreover, the classical substrates of caspase-3, poly-ADP ribose polymerase (PARP) was degraded after U937 cells treatment with NRP In addition, NRP increased the ratio of Bax/Bcl-2 protein expression. Although p38 inhibitor (SB203580) and ERK inhibitor (PD98059) failed to block cell death, JNK inhibitor (SP600125) had marked inhibitory effects on NRP -induced apoptosis. Furthermore, the phosphorylation of JNK was up-regulated, suggesting that JNK was responsible for NRP -induced apoptosis in U937 cells. These results suggested that the caspase, mitochondria and MAPK signal pathways were involved in NRP-induced U937 apoptosis.

• 出版日期2007-5
• 单位中国人民解放军第二一０医院; 沈阳药科大学