Attenuating effects of Granulocyte-colony stimulating factor (G-CSF) in radiation induced intestinal injury in mice

作者：Kim Joong Sun^*; Ryoo Seung Bum; Heo Kyu; Kim Joong Gook; Son Tae Gen; Moon Changjong; Yang Kwangmo

来源：Food and Chemical Toxicology, 2012, 50(9): 3174-3180.

DOI：10.1016/j.fct.2012.05.059

• G-CSF
• Radiation
• Intestinal injury
• Survival rate
• Crypt

Gastrointestinal injury is a major cause of death following exposure to high levels of radiation, and no effective treatments are currently available. In this study, we examined the capacity of granulocyte colony-stimulating factor (G-CSF) to mitigate intestinal injury in, and improve survival of, C3H/HeN mice given a lethal dose (12 Gy) of radiation to the abdomen. G-CSF (100 mu g/kg body weight) was injected subcutaneously daily for 3 days after irradiation and shown to improve survival and intestinal morphology at 3.5 days compared with saline-injected controls. The morphological features improved by G-CSF included crypt number and depth, villous length, and the length of basal lamina of 10 enterocytes. G-CSF also normalized the levels of circulating tumor necrosis factor alpha and attenuated the loss of peripheral neutrophils, caused by radiation-induced myelosuppression. In conclusion, our results suggest that G-CSF enhanced the survival of irradiated mice and minimized the effects of radiation on gastrointestinal injury.

• 出版日期2012-9