Assessment of microcirculation in a hind limb ischemia model

作者：Cai W J^*; Wang M J; Zhu Y C

来源：Chinese Pharmacological Bulletin, 2010, 26(5): 690-692.

• Angiography
• Animal model
• Fluorescent microspheres
• Hind limb ischemia
• Microcirculation
• Rats

Aim: To establish the standards for evaluation of microcirculation in a rat hind limb ischemia model. Methods: Rats were subjected to unilateral femoral artery ligation. After 4 weeks, the regional blood flow in skeletal muscles was measured by fluorescent microsphere assay. Formation of collateral vessels was determined by microangiography. Capillary density was examined by immunostaining for CD34. Results: In ischemic limbs, regional blood flow decreased significantly, some collateral vessels formed with typical "corkscrew" appearance, and histological analysis showed lower capillary density. Conclusion: Quantitative assessment of microcirculation in a hind limb ischemia model is established.

• 出版日期2010
• 单位复旦大学