科研之友
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摘要
A sensitive, selective, and reproducible reversed-phase high-performance liquid chromatography method coupled with electrospray ionization-ion trap mass spectrometry (LC–ESI–ITMS) was developed for the simultaneous quantification of pennogenin diglycoside (PD) in a small volume (100 L) of rat plasma. PD was extracted from rat plasma samples using liquid–liquid extraction with methanol, digoxin as internal standard. The chromatographic separation was performed on a reversed-phase Agilent C18 analytical column using a mobile phase of 0.1% formic acid solution–acetonitrile at a flow-rate of 1.0 mL/min. The mass spectrometer was operated in the negative ion mode at the deprotonated-molecular ions [M-H]– of parent drug. Calibration curve in spiked plasma was linear from . For the different samples with concentration of 0.50, 5.00, and L, recoveries of PD wererespectively . The intra-day assay relative standard deviation at , respectively. The interday assay %RSD at the previously mentioned concentrations were respectively. The method as successfully applied to the harmacokinetic study of PD in rats following either intravenous administration of PD solution or oral administration of the extract in Gongxuening capsules, a famous patent Chinese botanic drug.
