A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe

作者：Park Joon Soo; Baek Ahruem; Park Il Soo; Jun Bong Hyun; Kim Dong Eun^*

来源：Chemical Communications, 2013, 49(80): 9203-9205.

DOI：10.1039/c3cc45750h

We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.

• 出版日期2013