Antioxidants reduce TGF-beta2-induced gene expressions in human optic nerve head astrocytes

作者：Yu Alice L^*; Welge Lussen Ulrich

来源：Acta Ophthalmologica, 2013, 91(2): e92-e98.

DOI：10.1111/aos.12013

• antioxidant
• astrocyte
• heat shock protein
• optic nerve head

. Purpose: The goal of the present study was to investigate whether the antioxidants vitamin E, vitamin C and vitamin B1 can reduce the transforming growth factor-beta2 (TGF-2)-induced gene expressions in cultured human optic nerve head (ONH) astrocytes. Methods: Cultured human ONH astrocytes were pretreated with different concentrations of vitamin E, vitamin C and vitamin B1 and then exposed to 1.0ng/ml TGF-2 for 24hr. Expression of the heat shock proteins Hsp27 and B-crystallin, the extracellular matrix (ECM) component fibronectin and the ECM-modulating protein connective tissue growth factor (CTGF) was detected by immunohistochemistry or real-time PCR analysis. Results: TGF-2 increased the expression of Hsp27, B-crystallin, fibronectin and CTGF in human ONH astrocytes. Pretreatment with different concentrations of vitamin E, vitamin C and vitamin B1 reduced the TGF-2-stimulated gene expressions. Conclusion: In cultured human ONH astrocytes, the TGF-2-stimulated gene expressions could be reduced by pretreatment with vitamin E, vitamin C and vitamin B1. Therefore, the use of antioxidants in glaucomatous optic neuropathy might be a promising approach to prevent TGF-2-induced cellular changes in ONH astrocytes.

• 出版日期2013-3