Development of an analytical approach for identification and quantification of 5-alpha-androst-16-en-3-one in human milk

作者：Hartmann Constanze; Mayenzet Fabienne; Larcinese Jean Paul; Haefliger Olivier P; Buettner Andrea^*; Starkenmann Christian

来源：Steroids, 2013, 78(2): 156-160.

DOI：10.1016/j.steroids.2012.11.005

• Gel permeation chromatography
• GC-MS
• 5-alpha-Androst-16-en-3-one
• Breast milk
• Stable isotope dilution assay

A method was developed for the quantification of 5-alpha-androst-16-en-3-one in human breast milk based on application of a stable isotope dilution assay using 5 alpha-androst-16-en-3-one-6, 6-d(2). The procedure includes extraction of the human milk by hexane with subsequent clean-up of the obtained extract by gel permeation and silica gel column chromatography. The extracted samples were analyzed by gas chromatography-mass spectrometry. Using this method 5-alpha-androst-16-en-3-one could be identified and for the first time quantified in a concentration range of 26-155 ng/kg in human milk.

• 出版日期2013-2