A label-free electrochemical DNA biosensor based on covalent immobilization of salmonella DNA sequences on the nanoporous glassy carbon electrode

作者：Tabrizi Mahmoud Amouzadeh; Shamsipur Mojtaba^*

来源：Biosensors and Bioelectronics, 2015, 69: 100-105.

DOI：10.1016/j.bios.2015.02.024

• Nanoporous glassy carbon electrode
• Salmonella
• Covalent immobilization
• DNA biosensor
• Label-free detection

Herein, an easy and cost-effective approach to the immobilization of probe was performed. The amino modified salmonella ssDNA probe sequence was covalently linked with carboxylic group on the surface of nanoporous glassy carbon electrode to prepare the DNA biosensor. The differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques were used for the determination of salmonella DNA in the concentration ranges of 10-400 pM and 1-400 pM with limits of detection of 2.1 pM and 0.15 pM, respectively.

• 出版日期2015-7-15