Mouse Hemokinin-1 Decapeptide Subjected to a Brain-specific Post-translational Modification

作者：Deliconstantinos Georgia^*; Barton Stephen; Soloviev Mikhail; Page Nigel

来源：In Vivo, 2017, 31(5): 991-998.

DOI：10.21873/invivo.11159

• Mouse hemokinin-1
• substance P
• tachykinins
• N-terminal acetylation
• anti-tachykinin antibodies
• RP-HPLC
• MALDI-ToF

Background/Aim: The tachykinin mouse hemokinin-1, expressed by the mouse Tac4 gene, produces either analgesia or nociception, interacting with the neurokinin 1 receptor. TAC4 precursor processing is not identical to the processing of the TAC1 precursor, for the release of substance P (amidated undecapeptide). The characterization of the mouse hemokinin-1 sequence was required. Materials and Methods: We developed antitachykinin- specific antibodies for the immunoaffinity purification of tachykinins. Results: Using MALDI-ToF, we identified mouse hemokinin-1 as an amidated decapeptide expressed in murine brain and periphery. Furthermore, we interestingly observed an additional mass peak corresponding to acetylated mouse hemokinin-1 and this post-translational modification is brain-specific, not detected in the periphery. Conclusion: We suggest that the N-terminal acetylation of the peptide provides greater potency for ligand-receptor interactions during neural cell signaling.

• 出版日期2017-10