Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction

作者：Ali M E^*; Hashim U; Mustafa S; Man Y B Che; Dhahi Th S; Kashif M; Uddin Md Kamal; Abd Hamid S B

来源：Meat Science, 2012, 91(4): 454-459.

DOI：10.1016/j.meatsci.2012.02.031

• Meatball formulation
• Double quenched TaqMan probe
• Halal and Kosher foods
• Threshold cycle
• Ground meat

A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 +/- 0.20 for the pork and negative results for the others. Analysis of beef meatballs with spiked pork showed the assay can determine 100-0.01% contaminated pork with 102% PCR efficiency, high linear regression (r(2)=0.994) and %26lt;= 6% relative errors. Residuals analysis revealed a high precision in all determinations. Random analysis of commercial meatballs from pork, beef, chicken, mutton and goat, yielded a Ct between 15.89 +/- 0.16 and 16.37 +/- 0.22 from pork meatballs and negative results from the others, showing the suitability of the assay to determine pork in commercial meatballs with a high accuracy and precision.

• 出版日期2012-8