A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human

作者：Vo Tommy V; Das Jishnu; Meyer Michael J; Cordero Nicolas A; Akturk Nurten; Wei Xiaomu; Fair Benjamin J; Degatano Andrew G; Fragoza Robert; Liu Lisa G; Matsuyama Akihisa; Trickey Michelle; Horibata Sachi; Grimson Andrew; Yamano Hiroyuki; Yoshida Minoru; Roth Frederick P; Pleiss Jeffrey A; Xia Yu; Yu Haiyuan

来源：Cell, 2016, 164(1-2): 310-323.

DOI：10.1016/j.cell.2015.11.037

Here, we present FissionNet, a proteome-wide binary protein interactome for S. pombe, comprising 2,278 high-quality interactions, of which similar to 50% were previously not reported in any species. Fission-Net unravels previously unreported interactions implicated in processes such as gene silencing and pre-mRNA splicing. We developed a rigorous network comparison framework that accounts for assay sensitivity and specificity, revealing extensive species-specific network rewiring between fission yeast, budding yeast, and human. Surprisingly, although genes are better conserved between the yeasts, S. pombe interactions are significantly better conserved in human than in S. cerevisiae. Our framework also reveals that different modes of gene duplication influence the extent to which paralogous proteins are functionally repurposed. Finally, cross-species interactome mapping demonstrates that coevolution of interacting proteins is remarkably prevalent, a result with important implications for studying human disease in model organisms. Overall, FissionNet is a valuable resource for understanding protein functions and their evolution.

• 出版日期2016-1-14
• 单位RIKEN; McGill