Analysis of stallion semiologic semen parameters

作者：Banaszewska Dorota^*; Andraszek Katarzyna; Szostek Malgorzata; Wojcik Ewa; Danielewicz Agata; Walczak Jedrzejowska Renata

来源：Medycyna Weterynaryjna, 2015, 71(9): 563-567.

• stallion
• sperm
• silver nitrate
• strict Tygerberg criteria

Routine semen examination is an important tool in diagnostics and fertility evaluation in humans and animals, both in vivo and in vitro. Among the most important parameters in semen evaluation are sperm motility and morphology. The aim of the study was a detailed morphological analysis of stallion sperm with particular attention to the parameters of the sperm head. The subject of the study was the sperm of Polish Half Bred stallions, isolated post mortem from the tail of the epididymis. The smears obtained were stained with silver nitrate Tygerberg's criteria, which most precisely characterize the sperm head, and were used in the morphological evaluation of the sperm. The evaluation was expanded to include characterization of the tail, particularly the midpiece. The silver nitrate staining used in the present study enables staining of sperm structures which are not visible when other methods of smear preparation are used. In further research this method will be compared with others routinely used in laboratories in order to determine the extent to which the staining method affects sperm morphology and artefact formation.

• 出版日期2015-9