科研之友
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摘要
Graphite oxide (GO) was prepared by the Hummer procedure, and can be dispersed to stable colloid solution by ultrasonic wave. The GO exhibited an absorption peak at 313nm, and a resonance Rayleigh scattering (RRS) peak at 490nm. In pH 4.6 HAc-NaAc buffer solution, human serum albumin (HSA) combined with GO probe to form large HSA-GO particles that caused the RRS peak increasing at 490nm. The increased RRS intensity was linear to HSA concentration in the range 0.50-200 mu g/mL. Thus, a new and simple RRS method was proposed for the determination of HSA in samples, with a recovery of 98.1-104%.
- 出版日期2013-11
- 单位广西师范大学
