Molecular Characterization of Divergent Strawberry Mild Yellow Edge Virus Isolates from Eastern Canada

作者：Bhagwat, Basdeo; Dickison, Virginia; Su, Li; Bernardy, Mike; Wiersma, Paul A.; Nie, Xianzhou^*; Xiang, Yu^*

来源：Journal of Phytopathology, 2016, 164(9): 691-696.

DOI：10.1111/jph.12444

• deep-sequencing
• genome sequence
• SMYEV
• Strawberry mild yellow edge virus

The complete genome sequences of four new isolates of strawberry mild yellow edge virus (SMYEV) were determined and analysed. The isolates, designated as AB41-01, AB41-02, NB1165 and NS26, were found from strawberry fields showing strawberry decline symptoms in eastern Canada. AB41-01 and AB41-02 were from a single-plant sample originating from Prince Edward Island, while NB1165 came from New Brunswick and NS26 from Nova Scotia. Nucleotide sequence identities are 95.8% between AB41-01 and NB1165, 99.6% between AB41-02 and NS26, and 84% between AB41-01/NB1165 and AB41-02/NS26. The four isolates share nucleotide sequence identities of 83.5-89.9% to two previously identified SMYEV isolates, namely MY18 and D7. Phylogenetic analysis indicates that the four Canadian isolates represented two new SMYEV strain types and the strain divergences were not likely from recombination events among all presently known SMYEV isolates.

• 出版日期2016-9
• 单位西北农林科技大学