Nanoapertures for AFM-based single-molecule force spectroscopy

作者：Heucke Stephan F; Puchner Elias M; Stahl Stefan W; Holleitner Alexander W; Gaub Hermann E^*; Tinnefeld Philip

来源：International Journal of Nanotechnology, 2013, 10(5-7): 607-619.

DOI：10.1504/IJNT.2013.053529

• single-molecule force spectroscopy
• zero-mode waveguide
• nanoaperture
• force-activated enzyme
• mechanoenzymatics
• titin kinase
• AFM
• nanotechnology
• single-molecule fluorescence

Simultaneous single-molecule force spectroscopy and microfluorescence binding measurements are often hampered by background fluorescence from the bulk. Zero-Mode Waveguides (ZMW) restrict the excited volume but require a special design, which allows the tip of the force probing cantilever to protrude into the nanoaperture. Here, we describe the design and fabrication of such ZMW and report the first experiments where binding of fluorescent adenosine triphosphate to the force-activated enzyme titin kinase was measured while the enzyme was subjected to mechanical forces.

• 出版日期2013