New integrated in vivo microdialysis-electrochemical device for determination of the neurotransmitter dopamine in rat striatum of freely moving rats

作者：Lin, Li^*; Cai, Yuepiao; Lin, Ruipo; Yu, Li; Song, Caiyong; Gao, Hongchang; Li, Xiaokun

来源：Microchimica Acta, 2011, 172(1-2): 217-223.

DOI：10.1007/s00604-010-0484-0

• In vivo microdialysis
• Microsensor
• Dopamine
• Overoxidized polypyrrole
• Multi-wall carbon nanotube

A new kind of device was developed based on integrating in-vivo microdialysis with electrochemical microsensing. It can be applied to in-vivo determination of dopamine in rat striatum of freely moving rats. The microsensor was fabricated by electrochemically depositing a film containing overoxidized polypyrrole and multi-wall carbon nanotubes onto a carbon fiber microelectrode. It causes efficient and selective electrocatalytic oxidation of dopamine and displays relatively good stability and lifetime. The oxidation currents for dopamine are linear in the concentration range from 5.0 nM to 10 mu M, and the detection limit (at S/N = 3) is 0.5 nM. The integrated device was successfully applied to assessing the impact of an endogenous donor of nitric oxide (sodium nitroprusside) on the concentration of dopamine in striatum of moving rats. This is of significance to understand the physiological basis of Parkinson's disease.

• 出版日期2011-2
• 单位温州医科大学