A strain-promoted alkyne-azide cycloaddition (SPAAC) reaction of a novel EpCAM aptamer-fluorescent conjugate for imaging of cancer cells

作者：Subramanian Nithya^*; Sreemanthula Jagadeesh Babu; Balaji Baghavathi; Kanwar Jagat R; Biswas Jyotirmay; Krishnakumar Subramanian

来源：Chemical Communications, 2014, 50(80): 11810-11813.

DOI：10.1039/c4cc02996h

For the first time, a novel EpCAM aptamer (SYL3C)-DIBO-AF594 fluorescent conjugate was synthesised by bioorthogonal chemistry utilizing a strain promoted alkyne-azide cycloaddition (copper free click) reaction (SPAAC). The ligation efficiency of SPAAC was improved by freeze-thaw cycles. The obtained conjugate showed target specific binding and aided in the imaging of various EpCAM positive cancer cell lines like MCF7, MDAMB453, Weri-RB1 and PC3.

• 出版日期2014
• 单位迪肯大学