Anatomical location and redistribution of G protein-coupled estrogen receptor-1 during the estrus cycle in mouse kidney and specific binding to estrogens but not aldosterone

作者：Cheng Shi Bin; Dong Jing; Pang Yefei; LaRocca Jessica; Hixon Mary; Thomas Peter^*; Filardo Edward J

来源：Molecular and Cellular Endocrinology, 2014, 382(2): 950-959.

DOI：10.1016/j.mce.2013.11.005

• GPER-1
• Aldosterone binding
• Estradiol binding
• Distal convoluted tubules
• Estrus cycle
• Mineralocorticoid receptor

Prior studies have linked renoprotective effects of estrogens to G-protein-coupled estrogen receptor-1 (GPER-1) and suggest that aldosterone may also activate GPER-1. Here, the role of GPER-1 in murine renal tissue was further evaluated by examining its anatomical distribution, subcellular distribution and steroid binding specificity. Dual immunofluorescent staining using position-specific markers showed that GPER-1 immunoreactivity primarily resides in distal convoluted tubules and the Loop of Henle (stained with Tamm-Horsfall Protein-1). Lower GPER-1 expression was observed in proximal convoluted tubules marked with megalin, and GPER-1 was not detected in collecting ducts. Plasma membrane fractions prepared from whole kidney tissue or HEK293 cells expressing recombinant human GPER-1 (HEK-GPER-1) displayed high-affinity, specific [H-3]-17 beta-estradiol ([H-3]-E2) binding, but no specific [H-3]-aldosterone binding. In contrast, cytosolic preparations exhibited specific binding to [H-3]-aldosterone but not to [H-3]-E2, consistent with the subcellular distribution of GPER-1 and mineralocorticoid receptor (MR) in these preparations. Aldosterone and MR antagonists, spironolactone and eplerenone, failed to compete for specific [H-3]-E2 binding to membranes of HEK-GPER-1 cells. Furthermore, aldosterone did not increase [S-35]-GTP-gamma S binding to membranes of HEK-GPER-1 cells, indicating that it is not involved in G protein signaling mediated through GPER-1. During the secretory phases of the estrus cycle, GPER-1 is upregulated on cortical epithelia and localized to the basolateral surface during proestrus and redistributed intracellularly during estrus. GPER-1 is down-modulated during luteal phases of the estrus cycle with significantly less receptor on the surface of renal epithelia. Our results demonstrate that GPER-1 is associated with specific estrogen binding and not aldosterone binding and that GPER-1 expression is modulated during the estrus cycle which may suggest a physiological role for GPER-1 in the kidney during reproduction.

• 出版日期2014-2-15