Direct electron transfer glucose biosensor based on glucose oxidase self-assembled on electrochemically reduced carboxyl graphene

作者：Liang, Bo; Fang, Lu; Yang, Guang; Hu, Yichuan; Guo, Xishan; Ye, Xuesong^*

来源：Biosensors and Bioelectronics, 2013, 43: 131-136.

DOI：10.1016/j.bios.2012.11.040

• Glucose sensor
• Carboxyl graphene
• Electrochemical reduction
• Direct electrochemistry

A glucose biosensor based on direct electron transfer of glucose oxidase (GOD) self-assembled on the surface of the electrochemically reduced carboxyl graphene (ERCGr) modified glassy carbon electrode has been reported. X-ray photoelectron spectroscopy (XPS) analyses of ERCGr indicate most of the oxygen-containing groups such as epoxy/ether groups and hydroxyl groups in the carboxyl graphene were eliminated, while carboxylic acid groups remained. GOD was immobilized on the ERCGr modified glassy carbon electrode via self-assembly. The cyclic voltammetric result of the electrode shows a pair of well-defined and quasi-reversible redox peaks with a formal potential of -0.467 V and a peak to peak separation of 49 mV, revealing that the direct electron transfer between GOD and the electrode has been achieved. The proposed biosensor exhibits a linear response to glucose concentrations ranging from 2 to 18 mM with a detection limit of 0.02 mM. Moreover, this facile, fast, environment-friendly and economical preparation strategy of ERCGr may be extended for the preparation of other graphene based enzyme electrode biosensors.

• 出版日期2013-5-15
• 单位浙江大学