DETERMINATION OF VARENICLINE BY CAPILLARY ZONE ELECTROPHORESIS

作者：Alhazmi Hassan A; Makeen Hafiz A; El Deeb Sami^*

来源：Digest Journal of Nanomaterials and Biostructures, 2013, 8(1): 295-300.

• varenicline
• capillary electrophoresis
• quality control
• smoking

A fast, sensitive and selective method for the detection and quantification of varenicline (VRC) in tablet dosage form was developed using capillary zone electrophoresis separation with DAD detector. The used capillary was uncoated standard bare fused-silica with 100 mu m I. D, 40 cm total length and 31.5 cm effective length. The electrophoretic separations were carried out in 50 mM sodium phosphate buffer (pH 2.5) under normal mode (30 kV). Samples were filtered through a 0.22 mu m CME membrane filter and then injected hydrodynamically at a pressure of 50 mbar for 10 s. The calibration curve of VRC was linear (r=0.998) over the concentration range 1-16 mu g/ml. The LOD and LOQ values were 0.2 and 0.6 mu g/ml, respectively. Finally, the method proved to be suitable for quality control studies.

• 出版日期2013-3