Development of a Dispersive Liquid-Liquid Microextraction Method Based on Solidification of a Floating Organic Drop for the Determination of Beta-Carotene in Human Serum

作者：Rahimi A^*; Hashemi P

来源：Journal of Analytical Chemistry, 2014, 69(4): 352-356.

DOI：10.1134/S1061934814040078

• dispersive liquid-iquid microextraction
• solidification of organic drop
• high performance liquid chromatography
• beta-carotene
• human plasma
• central composite design optimization

A dispersive liquid-liquid microextraction method based on solidification of a floating organic drop (DLLME-SFO) was developed for HPLC determination of beta-carotene in human serum. A narrow-neck glass tube was used for simple and rapid collection of the solidified organic phase from aqueous surface after its centrifugation and cooling in a water bath. Acetone and 2-decanol were used as the disperser and organic phases, respectively. Effects of salt concentration and phase volumes on the extraction of the analyte were optimized using a central composite design method. During the optimization, a spectrophotometric method was used for determinations. Under the optimized conditions, an extraction recovery of 99.0 +/- 2.4% was obtained for five replicated analyses of beta-carotene with an enrichment factor of 40. A detection limit of 0.08 mu g/mL was achieved. The proposed method was successfully applied to the determination of beta-carotene in human serum samples.

• 出版日期2014-4