H2O2 production by copper amine oxidase, a component of the ecto-apyrase (ATPase)-containing protein complex(es) in the pea cell wall, is regulated by an elicitor and a suppressor from Mycosphaerella pinodes

作者：Toyoda Kazuhiro^*; Yasunaga Eri; Niwa Masanobu; Ohwatari Yuko; Nakashima Atsushi; Inagaki Yoshishige; Ichinose Yuki; Shiraishi Tomonori

来源：Journal of General Plant Pathology, 2012, 78(5): 311-315.

DOI：10.1007/s10327-012-0399-1

• Blue native PAGE
• Cell wall-associated ecto-apyrase (ATPase)
• Copper amine oxidase
• Mycosphaerella pinodes
• Pea (Pisum sativum L.)
• Suppressor

Blue native PAGE analysis for cell wall proteins from pea epicotyls demonstrated that cell wall-associated ecto-apyrase (ATPase) formed a large protein complex(es) ranging from 450 to 900 kDa; one of the components of the complex was copper amine oxidase (CuAO), which catalyzes the oxidation of amines with the subsequent generation of ammonia and hydrogen peroxide. CuAO activity was coordinately regulated in vitro with ATP-hydrolyzing activity by an elicitor and a suppressor from Mycosphaerella pinodes. Moreover, treatment of cell wall proteins with the suppressor caused the appearance of the apyrase monomer. On the basis of these results, M. pinodes may target the apyrase-containing protein complex(es) of the host to attenuate cell wall-based, extracellular defense(s) including the production of hydrogen peroxide.

• 出版日期2012-9