Development of a Real-Time PCR Assay for Detecting and Quantifying Human Bocavirus 2

作者：Xu Zi qian; Cheng Wei xia; Li Bo wen; Li Jie; Lan Bei; Duan Zhao jun^*

来源：Journal of Clinical Microbiology, 2011, 49(4): 1537-1541.

DOI：10.1128/JCM.00196-10

Human bocavirus 2 (HBoV2) is a parvovirus that has been recently identified in stool samples from children. Any association between the virus and clinical disease is unclear. A rapid, reliable diagnostic method is necessary to address this issue. In this study, we developed a sensitive and specific HBoV2 quantitative real-time PCR assay that targets the HBoV2 NP-1 gene, based on the TaqMan method. The assay could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV2 genome, with a dynamic range of 8 log units (10(1) to 10(8) copies). A clinical evaluation detected HBoV2 in 85 (24.6%) of 345 children with gastroenteritis, with viral loads ranging from 1.67 x 10(2) to 4.27 x 10(9) copies per ml of stool specimen.

• 出版日期2011-4
• 单位中国疾病预防控制中心