Ulk1 over-expression in human gastric cancer is correlated with patients' T classification and cancer relapse

作者：Chen, Min-Bin; Ji, Xiao-Zhi; Liu, Yuan-Yuan; Zeng, Ping; Xu, Xin-Yu; Ma, Rong; Guo, Zheng-Dong; Lu, Jian-Wei^*; Feng, Ji-Feng^*

来源：Oncotarget, 2017, 8(20): 33704-33712.

DOI：10.18632/oncotarget.16734

• gastric cancer
• Ulk1
• survival
• T classification
• cancer relapse

Ulk1 is a key autophagy protein. Here, we tested expression and potential function of Ulk1 in human gastric cancer. Ulk1 mRNA and protein were significantly elevated in multiple fresh human gastric cancer tissues. Its level was relatively low in surrounding normal epithelial tissues. Ulk1 over-expression was also observed in several gastric cancer cell lines (AGS, HGC-27, and SNU601). Remarkably, Ulk1 knockdown by targeted-shRNA inhibited AGS gastric cancer cell survival and proliferation. On the other hand, exogenous Ulk1 over-expression could further promote AGS cell survival and proliferation. Immunohistochemistry (IHC) staining assay of 145 paraffin-embedded gastric cancer tissues showed that Ulk1 was over-expressed in majority (114 out of 145) of gastric cancer tissues. Importantly, high Ulk1 expression in gastric cancer was correlated with patients' T classification and cancer relapse. Together, we demonstrate that Ulk1 over-expression in human gastric cancer is pro-survival. Its over-expression is associated with patients' T classification and cancer relapse.

• 出版日期2017-5-16
• 单位南京医科大学; 徐州医科大学; 江苏大学