Determination of Protein, Fat, Starch, and Amino Acids in Foxtail Millet [Setaria italica (L.) Beauv.] by Fourier Transform Near-Infrared Reflectance Spectroscopy

作者：Yang, Xiu-Shi; Wang, Li-Li; Zhou, Xian-Rong; Shuang, Shao-Min; Zhu, Zhi-Hua; Li, Nan; Li, Yan; Liu, Fang; Liu, San-Cai; Lu, Ping; Ren, Gui-Xing^*; Dong, Chuan

来源：Food Science and Biotechnology, 2013, 22(6): 1495-1500.

DOI：10.1007/s10068-013-0243-1

• foxtail millet
• protein
• starch
• amino acid
• NIRS

Quantitative detection of protein, fat, starch, and amino acids in foxtail millet using Fourier transform near-infrared spectroscopy (NIRS) was investigated. Foxtail millet samples (n=259) were analyzed using NIRS. Spectral data were linearized with data from chemical analyses. Calibration models were established using a partial least-squares (PLS) algorithm with cross-validation. Optimized models were tested using external validation set samples with coefficients of determination in the external validation (R-val(2) of >0.90. Residual predictive deviation (RPD) values were nearly equal to or >2.5 for crude protein, alanine, aspartic acid, glutamic acid, isoleucine, leucine, and serine. However, for glycine, histidine, phenylalanine, proline, threonine, tyrosine, and valine, the R-val(2) values were >0.83 and RPD values were nearly equal to or >2.0. For crude fat, total starch, arginine, and lysine, the R-val(2) values were >0.70 and RPD values were >1.5. NIRS is a rapid determination tool for foxtail millet breeding, and for quality control.

• 出版日期2013-12
• 单位中国农业科学院; 山西大学