科研之友
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摘要
Measurement of the free subunit of human chorionic gonadotropin (free -hCG) in serum is useful for prenatal screening. Concentrations of free -hCG vary in different races. Conventional assays used for such measurements have limitations. We applied the AlphaLISA to measure levels of free -hCG in maternal serum during 820 weeks of gestation in women from southern China. Two anti-free -hCG antibodies were used: one was coated on AlphaLISA acceptor beads and the one was biotinylated. The assay also contained donor beads coated with streptavidin. The AlphaLISA assay detection limit was 0.11ng/mL, and the analytical range was 0.11200ng/mL. The intra- and interassay coefficients of variation were 1.32%2.50% and 3.44%5.45%, respectively. The correlation with commercial Eu3+-labeled free -HCG-TRFIA assay was good (y=1.045x+1.580, r2=0.978). Median levels of free -hCG in maternal serum at 820 weeks gestation were higher in women from southern China compared with those reported in women from other countries. The AlphaLISA for free -HCG could become the assay of choice for applications in clinical diagnostics. The established median value of free -HCG is helpful in clinical diagnosis specific for southern Chinese women.
- 出版日期2013-4-1
- 单位南方医科大学
