Application of quantitative H-1-NMR method to determination of paeoniflorin in Paeoniae radix

作者：Tanaka Rie; Yamazaki Marina; Hasada Keiko; Nagatsu Akito^*

来源：Journal of Natural Medicines, 2013, 67(3): 657-661.

DOI：10.1007/s11418-012-0711-6

• Paeoniflorin
• Monoterpene glycoside
• Quantitative H-1-NMR
• Paeoniae radix
• Reagent purity
• qHNMR

A quantitative H-1-NMR method (qHNMR) was used to measure paeoniflorin content in Paeoniae radix (dried root of Paeonia lactiflora), of which paeoniflorin is a major component. The purity of paeoniflorin was calculated from the ratio of the intensity of the H-9 signal at delta 5.78 ppm of paeoniflorin to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using bisphenol A of certified reference material (CRM) grade. The paeoniflorin content in 2 separate samples of Paeoniae radix was determined by qHNMR and was found to be 2.15 and 2.45 %. We demonstrated that this method is useful for quantitative analysis of crude drugs.

• 出版日期2013-7