Different sample treatment approaches for the analysis of T-2 and HT-2 toxins from oats-based media

作者：Medina Angel^*; Valle Algarra Francisco M; Jimenez Misericordia; Magan Naresh

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(23): 2145-2149.

DOI：10.1016/j.jchromb.2010.05.043

• Analysis
• Type A trichothecenes
• Diode array
• Cereals

A LC-DAD method is proposed for the determination of the T-2 and HT-2 toxins in cultures of Fusarium langsethiae in oat-based and other in vitro media. Test media consisted of freshly prepared milled oats to which T-2 and HT-2 toxin stock solutions were added. Different mixtures of extraction solvent (acetonitrile:water and methanol water), extraction times (30', 60' or 90') and drying methods were investigated. Results showed that extraction with methanol:water (80:20, v/v) for 90 min, drying with N(2) and subsequent analysis by LC-DAD was the fastest and most user friendly method for detecting HT-2 and T-2 toxins production by F. langsethiae strains grown on oat-based media at levels of 0.459 and 0.508 mg of toxin/kg of agar, respectively. The proposed method was used to investigate toxin production of 6 F. langsethiae strains from northern Europe and provided clear chromatograms with no interfering peaks in media with and without glycerol as water activity modifier.

• 出版日期2010-8-1