Anti-proliferative A549 human activity of Bupleurum scrozonerifolium in lung cancer cells in vitro and in vivo

作者：Cheng YL; Lee SC; Lin SZ; Chang WL^*; Chen YL; Tsai NM; Liu YC; Tzao C; Yu DS; Harn HJ

来源：Cancer Letters, 2005, 222(2): 183-193.

DOI：10.1016/j.canlet.2004.10.015

• Bupleurum scorzonerifolium
• lung cancer
• apoptosis
• caspases
• cell cycle
• tubulin

Nan-Chai-Hu, the root of Bupleurum scorzonerifolium, is a traditional Chinese herb used in treatment of liver diseases such as hepatitis and cirrhosis. We recently reported that the acetone extract of B. scorzonerifolium. (BS-AE) could inhibit proliferation and induce apoptosis in A549 human lung cancer cells. We further examined its anti-proliferative mechanisms and in vivo anticancer effect. Our results showed that BS-AE had the ability to cause cell cycle arrest in G2/M phase, inducing tubulin polymerization, and activating caspase-3 and -9 in A549 cells. BS-AE-induced apoptosis could be blocked by the broad caspase inhibitor z-VAD-fmk in majority. The result of in vivo study showed that BS-AE could suppress growth in A549 subcutaneous xenograft tumors. These results indicate that BS-AE exerts anti proliferative effects on A549 cells in vitro and in vivo, and prompted us to further evaluate and elucidate the chemical composition profile of BS-AE.

• 出版日期2005-5-26