Binding phenomena and fluorescence quenching. I: Descriptive quantum principles of fluorescence quenching using a supermolecule approach

作者：Callis Patrik R^*

来源：Journal of Molecular Structure, 2014, 1077: 14-21.

DOI：10.1016/j.molstruc.2014.04.050

• Quantum principles of fluorescence
• Quantum principles of quenching
• Static quenching
• Dynamic quenching

Principal aspects of fluorescence and quenching are placed on an equal footing consistent with microscopic quantum concepts familiar to all who use fluorescence in the study of association of ligands with proteins. Quenching of fluorophores involved in determination of ligand binding to proteins is described in terms of generic quantum principles using a framework in which the fluorophore and quencher are together considered a %26quot;supermolecule%26quot;. Quenching then becomes just another form of internal conversion, which in turn leads to new language for defining %26quot;dynamic%26quot; and %26quot;static%26quot; quenching, for which there exist disparate definitions. The benefit of casting quenching in this manner, and citing relevant literature, has been to expand the vocabulary and mental imagery associated with quenching.

• 出版日期2014-12-5