Antiviral activity of Distictella elongata (Vahl) Urb. (Bignoniaceae), a potentially useful source of anti-dengue drugs from the state of Minas Gerais, Brazil

作者：Simoes L R; Maciel G M; Brandao G C; Kroon E G; Castilho R O^*; Oliveira A B

来源：Letters in Applied Microbiology, 2011, 53(6): 602-607.

DOI：10.1111/j.1472-765X.2011.03146.x

• acacetin-7-O-rutinoside
• antiviral activity
• Bignoniaceae
• DENV-2
• Distictella elongata
• pectolinarin
• triterpenes

Aims: To investigate the in vitro antiviral activity of Distictella elongata (Vahl) Urb. ethanol extracts from leaves (LEE), fruits (FEE), stems and their main components.
Methods and Results: The antiviral activity was evaluated against human herpesvirus type 1 (HSV-1), murine encephalomyocarditis virus (EMCV), vaccinia virus Western Reserve (VACV-WR) and dengue virus 2 (DENV-2) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. LEE presented anti-HSV-1 [EC(50) 142.8 +/- 5.3 mu g ml(-1); selectivity index (SI) 2 0] and anti-DENV-2 activity (EC(50) 9.8 +/- 1.3 mu g ml(-1); SI 1.5). The pectolinarin (1) isolated from LEE was less active against HSV-1 and DENV-2. A mixture of the triterpenoids ursolic, pomolic and oleanolic acids was also obtained. Ursolic and oleanolic acids have shown antiviral activity against HSV1. A mixture of pectolinarin (1) and acacetin-7-O-rutinoside (2) was isolated from FEE and has presented anti-DENV-2 activity (EC(50) 11.1 +/- 1.6 mu g ml(-1); SI > 45). Besides the antiviral activity, D. elongata has disclosed antioxidant effect.
Conclusions: These data shows that D. elongata has antiviral activity mainly against HSV-1 and DENV-2, besides antioxidant activity. These effects might be principally attributed to flavonoids isolated.
Significance and Impact of the Study: Distictella elongata might be considered a promising source of anti-dengue fever phytochemicals.

• 出版日期2011-12