Genetic diversity among Bacillus thuringiensis isolates from Iran based on random amplified polymorphic DNA (RAPD) marker

作者：Chalajour Hengameh^*; Dolatabad Saeed Safari; Jouzani Gholamreza Salehi; Jouzani Salehi

来源：Research on Crops, 2013, 14(3): 945-949.

• Biological control
• Bt strain
• genetic diversity
• RAPD marker

Biological control of plant-parasitic nematodes has long been considered as an alternative to nematicides. In this study, RAPD-PCR marker was conducted to study genetic diversity among 36 local isolates. Microscopic studies showed that about 87% of isolates contained more than one type of crystals. Also, genetic diversity of strains was studied by RAPD marker. The number of amplicons produced in various primers was 202 polymorphic. The number of amplified polymorphic bands ranged from 12 in OPJ-og primer to 77 in OPT-14 primer, with a total of 2190 polymorphic bands. The dendrogram showed three major groups. The first group clustered Bt11, Bt9AA1, Bt4Q and Bt73 local isolates. In the second major group, 10 local isolates were clustered together. The most tested strains were clustered in the third group (22 strains). Our analysis results showed that levels of similarity among the strains of the various locations were greater than the levels of similarity between the strains collected from one location.

• 出版日期2013-9