A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model

作者：Luo Yuzi; Terkawi Mohamad Alaa; Jia Honglin; Aboge Gabriel Oluga; Goo Youn Kyoung; Cao Shinuo; Li Yan; Yu Longzheng; Ooka Hideo; Kamyingkird Ketsarin; Masatani Tatsunori; Zhang Shoufa; Nishikawa Yoshifumi; Igarashi Ikuo; Xuan Xuenan^*

来源：Experimental Parasitology, 2012, 130(2): 178-182.

DOI：10.1016/j.exppara.2011.10.012

• Babesia microti
• Secreted antigen
• BmSA1
• Double antibody sandwich ELISA

A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) targeting secreted antigen 1 of Babesia microti (BmSA1) was developed for detection of B. microti infection. The optimized DAS-ELISA was sensitive enough to detect circulating BmSA1 by day 2 post-infection, in sequential sera of a hamster infected with B. microti. This detection was 4 days earlier than antibody detection by indirect ELISA. The kinetics of circulating BmSA1 coincided with the profile of parasitemia. The specificity of this assay was evaluated using sera from animals experimentally infected with different species of Babesia. The DAS-ELISA had a higher sensitivity than the microscopic examination of Giemsa-stained blood smears for detection of the infection in hamsters. Taken together, these results indicated that BmSA1 could be a potential marker for surveillance of human babesiosis.

• 出版日期2012-2
• 单位延边大学