Extraction of soluble arabinoxylan from enzymatically pretreated wheat bran and production of short xylo-oligosaccharides and arabinoxylo-oligosaccharides from arabinoxylan by glycoside hydrolase family 10 and 11 endoxylanases

作者：Mathew Sindhu^*; Karlsson Eva Nordberg; Adlercreutz Patrick

来源：Journal of Biotechnology, 2017, 260: 53-61.

DOI：10.1016/j.jbiotec.2017.09.006

• Xylo-oligosaccharides
• Prebiotics
• Enzymes
• Arabinoxylan
• Monosugars

The enzymatic, ecofriendly pretreatment of wheat bran with a-amylase from Bacillus amyloliquifaciens or B. licheniformis at 90 degrees C for 1.5 h followed by Neutrase at 50 degrees C for 4 h, aqueous liquefaction at 121 degrees C for 15 h and ethanol precipitation enabled the production of soluble arabinoxylan (AX) with purity of 70.9% and 68.4% (w/w) respectively. Process alternatives tried, to simplify the process and curtail the cost resulted in AX products with different purities, yields and arabinose to xylose ratio (A/X). Among the two glycoside hydrolase (GH) family endoxylanases evaluated, GH10 family hydrolysed soluble AX more efficiently with xylanase from Geobacillus stearothermophilus T-6 (GsXyn10A) producing maximum amount of quantifiable short xylo-oligosaccharides (XOS) and arabinoxylo-oligosaccharides (AXOS) (53% w/w) followed by the catalytic module of Rhodothermus marinus Xyn10A (RmXyn10A-CM) with 37% (w/w) conversion. The GH11 family endoxylanases, from Thermomyces lanuginosus (Pentopan Mono BG (TM)) and Neocallimastix patriciarum (NpXyn11A) gave conversions of 21% and 22% (w/w) of the soluble AX, respectively (major AXOS products were not quantified). In addition to the XOS formed such as X-2, X-3 and X-4, the AXOS products identified were A(3)X and A(2)XX in the case of GsXyn10A and RmXyn10A-CM while Pentopan Mono BG and NpXyn11A produced XA(3)XX as the major AXOS product.

• 出版日期2017-10-20