Anti-GM1 antibodies affect the integrity of lipid rafts

作者：Ueda Akihiro; Shima Sayuri; Miyashita Tadayuki; Ito Shinji; Ueda Masami; Kusunoki Susumu; Asakura Kunihiko; Mutoh Tatsuro^*

来源：Molecular and Cellular Neuroscience, 2010, 45(4): 355-362.

DOI：10.1016/j.mcn.2010.07.008

• Guillain-Barre syndrome
• Anti GM1 antibody
• Trk neurotrophin receptor
• Nerve growth factor
• Lipid rafts

Previous studies have shown that patients with the axonal form of Guillain-Barre syndrome (CBS) develop autoantibodies against GM1 ganglioside (GM1) Nerve growth factor (NGF) is essential for neuronal survival in vivo and its functional receptor is Trk-tyrosine kinase Here we examined the biological effects of sera from patients with the axonal form of GBS on the morphology and the phosphorylation state of Trk-tyrosine kinase in PC12 cells Furthermore we examined the effect of the sera on the integrity of membrane lipid rafts biochemically The data show that anti GM1 antibodies found in patients sera but not control sera inhibit NGF-induced Trk autophosphorylation Most intriguingly the autoantibodies alter the distribution of Trk in lipid rafts without shifting the distribution of a rafts marker protein These data strongly suggest that anti GM1 a

• 出版日期2010-12