Allogeneic virus-specific T cells with HLA alloreactivity do not produce GVHD in human subjects

作者：Melenhorst J Joseph; Leen Ann M; Bollard Catherine M; Quigley Maire F; Price David A; Rooney Cliona M; Brenner Malcolm K; Barrett A John; Heslop Helen E^*

来源：Blood, 2010, 116(22): 4700-4702.

DOI：10.1182/blood-2010-06-289991

Adoptive transfer of viral antigen-specific memory T cells can reconstitute antiviral immunity, but in a recent report a majority of virus-specific cytotoxic T-lymphocyte (CTL) linesshowedin vitro cross-reactivity against allo-human leukocyte antigen (HLA) molecules as measured by interferon-gamma secretion. We therefore reviewed our clinical experience with adoptive transfer of allogeneic hematopoietic stem cell transplantation donor-derived virus-specific CTLs in 153 recipients, including 73 instances where there was an HLA mismatch. There was no de novo acute graft-versus-host disease after infusion, and incidence of graft-versus-host disease reactivation was low and not significantly different in recipients of matched or mismatched CTL. However, we found that virus-specific T cell lines recognized up to 10% of a panel of 44 HLA disparate targets, indicating that virus-specific T cells can have cross-reactivity with HLA-mismatched targets in vitro. These data indicate that the adoptive transfer of partially HLA-mismatched virus-specific CTL is safe despite in vitro recognition of recipient HLA molecules. (Blood. 2010; 116(22): 4700-4702)

• 出版日期2010-11-25