Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex

作者：Prieto Marcela B; Georg Raphaela C; Gonzales Zubiate Fernando A; Luz Juliana S; Oliveira Carla C^*

来源：BMC Molecular Biology, 2015, 16(1): 7.

DOI：10.1186/s12867-015-0037-5

• box C/D snoRNP assembly
• R2TP complex
• Nop17

Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described.
Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2.
Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits.

• 出版日期2015-3-18